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cell lines hek 293 t atcc crl  (ATCC)


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    Structured Review

    ATCC cell lines hek 293 t atcc crl
    Cell Lines Hek 293 T Atcc Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 38400 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lines+hek+293+t+atcc+crl/293T/pm41950924-749-142-147
    Average 99 stars, based on 38400 article reviews
    cell lines hek 293 t atcc crl - by Bioz Stars, 2026-10
    99/100 stars

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    Recombinant:


    Article Title: Directed evolution of APOX for proximity labeling using phenols with high redox potentials.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken α-c-Myc MilliporeSigma Cat#AB3252 Goat AlexaFluor647-αCh ThermoFisher Cat#A-21449 Streptavidin-Phycoerythrin (SA-PE) ThermoFisher Cat#S886 Streptavidin-AlexaFluor568 (SA-A568) ThermoFisher Cat#S11226 Streptavidin-HRP (SA-HRP) ThermoFisher Cat#S911 Rabbit-αFlag Sigma Cat#F7425 Goat AlexaFluor488-αRabbit ThermoFisher Cat#A11008 mouse anti-BSA Sigma Cat#SAB4200688-100UL goat anti-mouse HRP conjugate Sigma Cat#AP124P Streptavidin-AlexaFluor594 (SA-A594) Invitrogen Cat#S11227 Bacterial and virus strains DH5α E.coli Invitrogen Cat#12034013 Saccharomyces cerevisiae strain BJ5465 ATCC Cat#208289 Biological samples Cortical neuronal cultures from prenatal Sprague-Dawley rats Envigo https://www.inotiv.com/hubfs/resources/ data-sheets/envigo-44-sprague-dawleymodel-sheet-cardiology-a4_screen.pdf Chemicals, peptides, and recombinant proteins Biotin-PEG3-azide ThermoFisher Cat#50-210-7600 Desthiobiotin-PEG3-azide Sigma Cat#902020 200 μL PierceTM Streptavidin Agarose beads Thermo Cat#20349 Carbonic anhydrase from bovine erythrocytes Sigma Cat#C3934-100MG Horseradish peroxidase Biosynth Cat#FP159434 phenosafranin Sigma Cat#199648-1G Nile blue Sigma Cat#370088 Critical commercial assays Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat#D2004 Deposited data mass spectrometry proteomics data ProteomeXchange Consortium via the PRIDE partner repository PXD060753 and 10.6019/PXD060753 https://www.ebi.ac.uk/pride/archive/ projects/PXD060753 Experimental models: Cell lines HEK 293 T ATCC CRL-3216 Recombinant DNA FUGW 3rd generation lentiviral plasmid Lois et al.81 Addgene plasmid #14883 Oligonucleotides Con2.Fwd (5′-CTAGTGGTGGAGGAGGCTCTGG TGGAGGCGGTAGCGGAGGCGGAGGGTCGGCTAG-3′) Integrated DNA Technologies N/A Con2.Rev (5′-TATCAGATCTCGAGCTATTACAAGTCCT CTTCAGAAATAAGCTTTTGTTCGGATCC-3′) Integrated DNA Technologies N/A HomR.Fwd (5′-CAAGGTCTGCAGGCTAGTGG TGGAGGAGGCTCTGGTG-3′) Integrated DNA Technologies N/A HomR.Rev (5′-CTACACTGTTGTTATCAGATC TCGAGCTATTACAAGTC-3′) Integrated DNA Technologies N/A (Continued on next page) ll OPEN ACCESS Technology e1 Cell Chemical Biology 33, 1–15.e1–e13, April 16, 2026 .. HEK 293 T cells from ATCC (CRL-3216, passage number < 25, female origin, authenticated using STR profiling and mycoplasma testing) were cultured as a monolayer in DMEM (ATCC) supplemented with 10% (v/v) FBS (Corning) and 2 mM L-glutamine (ATCC) (DMEM-FBS) at 37 ◦C under 5% CO2.

    Virus:

    Article Title: Directed evolution of APOX for proximity labeling using phenols with high redox potentials.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken α-c-Myc MilliporeSigma Cat#AB3252 Goat AlexaFluor647-αCh ThermoFisher Cat#A-21449 Streptavidin-Phycoerythrin (SA-PE) ThermoFisher Cat#S886 Streptavidin-AlexaFluor568 (SA-A568) ThermoFisher Cat#S11226 Streptavidin-HRP (SA-HRP) ThermoFisher Cat#S911 Rabbit-αFlag Sigma Cat#F7425 Goat AlexaFluor488-αRabbit ThermoFisher Cat#A11008 mouse anti-BSA Sigma Cat#SAB4200688-100UL goat anti-mouse HRP conjugate Sigma Cat#AP124P Streptavidin-AlexaFluor594 (SA-A594) Invitrogen Cat#S11227 Bacterial and virus strains DH5α E.coli Invitrogen Cat#12034013 Saccharomyces cerevisiae strain BJ5465 ATCC Cat#208289 Biological samples Cortical neuronal cultures from prenatal Sprague-Dawley rats Envigo https://www.inotiv.com/hubfs/resources/ data-sheets/envigo-44-sprague-dawleymodel-sheet-cardiology-a4_screen.pdf Chemicals, peptides, and recombinant proteins Biotin-PEG3-azide ThermoFisher Cat#50-210-7600 Desthiobiotin-PEG3-azide Sigma Cat#902020 200 μL PierceTM Streptavidin Agarose beads Thermo Cat#20349 Carbonic anhydrase from bovine erythrocytes Sigma Cat#C3934-100MG Horseradish peroxidase Biosynth Cat#FP159434 phenosafranin Sigma Cat#199648-1G Nile blue Sigma Cat#370088 Critical commercial assays Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat#D2004 Deposited data mass spectrometry proteomics data ProteomeXchange Consortium via the PRIDE partner repository PXD060753 and 10.6019/PXD060753 https://www.ebi.ac.uk/pride/archive/ projects/PXD060753 Experimental models: Cell lines HEK 293 T ATCC CRL-3216 Recombinant DNA FUGW 3rd generation lentiviral plasmid Lois et al.81 Addgene plasmid #14883 Oligonucleotides Con2.Fwd (5′-CTAGTGGTGGAGGAGGCTCTGG TGGAGGCGGTAGCGGAGGCGGAGGGTCGGCTAG-3′) Integrated DNA Technologies N/A Con2.Rev (5′-TATCAGATCTCGAGCTATTACAAGTCCT CTTCAGAAATAAGCTTTTGTTCGGATCC-3′) Integrated DNA Technologies N/A HomR.Fwd (5′-CAAGGTCTGCAGGCTAGTGG TGGAGGAGGCTCTGGTG-3′) Integrated DNA Technologies N/A HomR.Rev (5′-CTACACTGTTGTTATCAGATC TCGAGCTATTACAAGTC-3′) Integrated DNA Technologies N/A (Continued on next page) ll OPEN ACCESS Technology e1 Cell Chemical Biology 33, 1–15.e1–e13, April 16, 2026 .. HEK 293 T cells from ATCC (CRL-3216, passage number < 25, female origin, authenticated using STR profiling and mycoplasma testing) were cultured as a monolayer in DMEM (ATCC) supplemented with 10% (v/v) FBS (Corning) and 2 mM L-glutamine (ATCC) (DMEM-FBS) at 37 ◦C under 5% CO2.

    Plasmid Preparation:

    Article Title: Directed evolution of APOX for proximity labeling using phenols with high redox potentials.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken α-c-Myc MilliporeSigma Cat#AB3252 Goat AlexaFluor647-αCh ThermoFisher Cat#A-21449 Streptavidin-Phycoerythrin (SA-PE) ThermoFisher Cat#S886 Streptavidin-AlexaFluor568 (SA-A568) ThermoFisher Cat#S11226 Streptavidin-HRP (SA-HRP) ThermoFisher Cat#S911 Rabbit-αFlag Sigma Cat#F7425 Goat AlexaFluor488-αRabbit ThermoFisher Cat#A11008 mouse anti-BSA Sigma Cat#SAB4200688-100UL goat anti-mouse HRP conjugate Sigma Cat#AP124P Streptavidin-AlexaFluor594 (SA-A594) Invitrogen Cat#S11227 Bacterial and virus strains DH5α E.coli Invitrogen Cat#12034013 Saccharomyces cerevisiae strain BJ5465 ATCC Cat#208289 Biological samples Cortical neuronal cultures from prenatal Sprague-Dawley rats Envigo https://www.inotiv.com/hubfs/resources/ data-sheets/envigo-44-sprague-dawleymodel-sheet-cardiology-a4_screen.pdf Chemicals, peptides, and recombinant proteins Biotin-PEG3-azide ThermoFisher Cat#50-210-7600 Desthiobiotin-PEG3-azide Sigma Cat#902020 200 μL PierceTM Streptavidin Agarose beads Thermo Cat#20349 Carbonic anhydrase from bovine erythrocytes Sigma Cat#C3934-100MG Horseradish peroxidase Biosynth Cat#FP159434 phenosafranin Sigma Cat#199648-1G Nile blue Sigma Cat#370088 Critical commercial assays Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat#D2004 Deposited data mass spectrometry proteomics data ProteomeXchange Consortium via the PRIDE partner repository PXD060753 and 10.6019/PXD060753 https://www.ebi.ac.uk/pride/archive/ projects/PXD060753 Experimental models: Cell lines HEK 293 T ATCC CRL-3216 Recombinant DNA FUGW 3rd generation lentiviral plasmid Lois et al.81 Addgene plasmid #14883 Oligonucleotides Con2.Fwd (5′-CTAGTGGTGGAGGAGGCTCTGG TGGAGGCGGTAGCGGAGGCGGAGGGTCGGCTAG-3′) Integrated DNA Technologies N/A Con2.Rev (5′-TATCAGATCTCGAGCTATTACAAGTCCT CTTCAGAAATAAGCTTTTGTTCGGATCC-3′) Integrated DNA Technologies N/A HomR.Fwd (5′-CAAGGTCTGCAGGCTAGTGG TGGAGGAGGCTCTGGTG-3′) Integrated DNA Technologies N/A HomR.Rev (5′-CTACACTGTTGTTATCAGATC TCGAGCTATTACAAGTC-3′) Integrated DNA Technologies N/A (Continued on next page) ll OPEN ACCESS Technology e1 Cell Chemical Biology 33, 1–15.e1–e13, April 16, 2026 .. HEK 293 T cells from ATCC (CRL-3216, passage number < 25, female origin, authenticated using STR profiling and mycoplasma testing) were cultured as a monolayer in DMEM (ATCC) supplemented with 10% (v/v) FBS (Corning) and 2 mM L-glutamine (ATCC) (DMEM-FBS) at 37 ◦C under 5% CO2.

    Mass Spectrometry:

    Article Title: Directed evolution of APOX for proximity labeling using phenols with high redox potentials.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken α-c-Myc MilliporeSigma Cat#AB3252 Goat AlexaFluor647-αCh ThermoFisher Cat#A-21449 Streptavidin-Phycoerythrin (SA-PE) ThermoFisher Cat#S886 Streptavidin-AlexaFluor568 (SA-A568) ThermoFisher Cat#S11226 Streptavidin-HRP (SA-HRP) ThermoFisher Cat#S911 Rabbit-αFlag Sigma Cat#F7425 Goat AlexaFluor488-αRabbit ThermoFisher Cat#A11008 mouse anti-BSA Sigma Cat#SAB4200688-100UL goat anti-mouse HRP conjugate Sigma Cat#AP124P Streptavidin-AlexaFluor594 (SA-A594) Invitrogen Cat#S11227 Bacterial and virus strains DH5α E.coli Invitrogen Cat#12034013 Saccharomyces cerevisiae strain BJ5465 ATCC Cat#208289 Biological samples Cortical neuronal cultures from prenatal Sprague-Dawley rats Envigo https://www.inotiv.com/hubfs/resources/ data-sheets/envigo-44-sprague-dawleymodel-sheet-cardiology-a4_screen.pdf Chemicals, peptides, and recombinant proteins Biotin-PEG3-azide ThermoFisher Cat#50-210-7600 Desthiobiotin-PEG3-azide Sigma Cat#902020 200 μL PierceTM Streptavidin Agarose beads Thermo Cat#20349 Carbonic anhydrase from bovine erythrocytes Sigma Cat#C3934-100MG Horseradish peroxidase Biosynth Cat#FP159434 phenosafranin Sigma Cat#199648-1G Nile blue Sigma Cat#370088 Critical commercial assays Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat#D2004 Deposited data mass spectrometry proteomics data ProteomeXchange Consortium via the PRIDE partner repository PXD060753 and 10.6019/PXD060753 https://www.ebi.ac.uk/pride/archive/ projects/PXD060753 Experimental models: Cell lines HEK 293 T ATCC CRL-3216 Recombinant DNA FUGW 3rd generation lentiviral plasmid Lois et al.81 Addgene plasmid #14883 Oligonucleotides Con2.Fwd (5′-CTAGTGGTGGAGGAGGCTCTGG TGGAGGCGGTAGCGGAGGCGGAGGGTCGGCTAG-3′) Integrated DNA Technologies N/A Con2.Rev (5′-TATCAGATCTCGAGCTATTACAAGTCCT CTTCAGAAATAAGCTTTTGTTCGGATCC-3′) Integrated DNA Technologies N/A HomR.Fwd (5′-CAAGGTCTGCAGGCTAGTGG TGGAGGAGGCTCTGGTG-3′) Integrated DNA Technologies N/A HomR.Rev (5′-CTACACTGTTGTTATCAGATC TCGAGCTATTACAAGTC-3′) Integrated DNA Technologies N/A (Continued on next page) ll OPEN ACCESS Technology e1 Cell Chemical Biology 33, 1–15.e1–e13, April 16, 2026 .. HEK 293 T cells from ATCC (CRL-3216, passage number < 25, female origin, authenticated using STR profiling and mycoplasma testing) were cultured as a monolayer in DMEM (ATCC) supplemented with 10% (v/v) FBS (Corning) and 2 mM L-glutamine (ATCC) (DMEM-FBS) at 37 ◦C under 5% CO2.



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